We used the Solvita IRTH tool to measure the real-time impact of various soil treatments on microbial activity. The alternative treatments considered were Chandler Soil (red line, 16 ounces per acre), commercial molasses (orange line, 2 gallons per acre), and humic acid (brown line, 2 gallons per acre). Chandler Soil generated the fastest microbial response (less than two hours after treatment), and the soil sample treated with Chandler Soil produced 70% more CO2 than molasses and 5.5 times more CO2 than humic acid after just 2.5 days.